# 196: DESULFOCAPSA SULFEXIGENS MEDIUM


## Main sol. 196

961	ml	**Solution A**	
30	ml	**Solution B**	
10	ml	**Solution C**	

1. First dissolve 2.7 g FeCl3 x 6 H2O in distilled water and adjust the pH to 7.0 with 1 N NaOH (about 3 ml). Add and dissolve remaining salts of solution A and sparge medium with 80% N2 and 20% CO2 gas mixture for at least 30 min, then distribute under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere. Solution C is autoclaved separately under 100% N2 gas atmosphere. To complete the medium appropriate amounts of solutions B and C are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be adjusted to 7.2.
2. Note: For transfers use 5 - 10% (v/v) inoculum.


## Solution A

2.7	g	FeCl3 x 6 H2O	
3	g	Na2SO4	
0.2	g	KH2PO4	
0.3	g	NH4Cl	
21	g	NaCl	
3	g	MgCl2 x 6 H2O	
0.5	g	KCl	
0.15	g	CaCl2 x 2 H2O	
1	ml	**Trace element solution SL-10**	
960	ml	Distilled water	


## Solution B

1.5	g	Na2CO3	
30	ml	Distilled water	


## Solution C

2.5	g	Na2S2O3 x 5 H2O	
10	ml	Distilled water	


## Trace element solution SL-10

10	ml	HCl (25%)	
1.5	g	FeCl2 x 4 H2O	
70	mg	ZnCl2	
100	mg	MnCl2 x 4 H2O	
6	mg	H3BO3	
190	mg	CoCl2 x 6 H2O	
2	mg	CuCl2 x 2 H2O	
24	mg	NiCl2 x 6 H2O	
36	mg	Na2MoO4 x 2 H2O	
990	ml	Distilled water	

1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.