# 2: BACILLUS PASTEURII MEDIUM


## Main sol. 2

1000	ml	**Main sol. 1**	
20	g/l	Urea	

1. To medium 1 add 20 g/l urea before autoclaving. Do not adjust pH; pH raises to about 8 due to heat degraded urea.
2. For sporulation enhancement add 10 mg/l MnSO4 x H2O.


## Main sol. 1

5	g	Peptone	
3	g	Meat extract	
15	g	Agar, for solid medium	
1000	ml	Distilled water	

1. Adjust pH to 7.0.
2. For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.