# 205: NATRONOBACTERIUM MEDIUM


## Main sol. 205

15	g	Casamino acids	
3	g	Trisodium citrate x 2 H2O	
2.5	g	Glutamic acid	
2.5	g	MgSO4 x 7 H2O	
2	g	KCl	
250	g	NaCl	
20	g	Agar	
1000	ml	Distilled water	

1. Dissolve agar by heating before adding sodium chloride. Adjust pH to 7.0 before autoclaving. Adjust pH to 8.5 with sterile 5% Na2CO3 after heat sterilization of the medium.