# 215c: BHI MEDIUM FOR STRICT ANAEROBES


## Main sol. 215c

37	g	Brain heart infusion (DIFCO)	
0.25	g	L-Cysteine HCl x H2O	
0.25	g	Na2S x 9 H2O	
1000	ml	Distilled water	

1. Prepare medium anoxically under 100% N2 gas atmosphere. Reducing agents are added after autoclaving from sterile anoxic solutions prepared under N2.