# 219: MYCOBACTERIUM MEDIUM


## Main sol. 219

2	g	Yeast extract	
2	g	Proteose peptone no. 3	
2	g	Casein peptone (tryptic digested)	
2.5	g	Na2HPO4 x 12 H2O	
1	g	KH2PO4	
1.5	g	Sodium citrate	
0.6	g	MgSO4 x 7 H2O	
50	ml	Glycerol	
0.5	g	Tween	
20	g	Agar	
1000	ml	Distilled water	

1. Adjust pH to 7.0.