# 221: AZOSPIRILLUM MEDIUM


## Main sol. 221

0.05	g	Yeast extract	
0.25	g	K2HPO4	
0.01	g	FeSO4 x 7 H2O	
1	mg	Na2MoO4 x 2 H2O	
2	mg	MnSO4 x H2O	
0.2	g	MgSO4 x 7 H2O	
0.1	g	NaCl	
0.02	g	CaCl2 x 2 H2O	
1	g	(NH4)2SO4	
0.1	mg	Biotin	
1.5	%	Agar, if required	
950	ml	Distilled water	
25	ml	Glucose (filter-sterilized, 20 %)	
25	ml	Na malate (filter-sterilized, 20 %)	

1. Adjust pH of the medium to 7.1. Add 1.5% agar if needed. Autoclave for 15 min at 121°C. After sterilization add 25 ml each of filter-sterilized 20% glucose and 20% Na-malate.