# 232: MRS MEDIUM WITH CYSTEINE ## Main sol. 232 10 g Casein peptone (tryptic digest) 10 g Meat extract 5 g Yeast extract 20 g Glucose 1 g Tween 80 2 g K2HPO4 5 g Na-acetate 2 g (NH4)2 citrate 0.2 g MgSO4 x 7 H2O 0.05 g MnSO4 x H2O 1000 ml Distilled water 1. Adjust pH to 6.2 - 6.5. 2. Add 0.05% cysteine-hydrochloride.