# 241: GLUTAMINE MEDIUM


## Main sol. 241

5	g	Peptone	
3	g	Meat extract	
15	g	Agar, if necessary	
100	mg/l	L-Glutamine	
100	mg/l	Tryptophan (filter-sterilized)	
1000	ml	Distilled water	

1. Adjust pH to 7.0. For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.