# 243: SEAWATER YEAST PEPTONE AGAR


## Main sol. 243

5	g	Peptone	
3	g	Yeast extract	
12	g	Agar	
250	ml	Distilled water	
750	ml	Sea water (filtered, aged)	

1. Adjust pH to 7.8. Boil for 5 min, filter and readjust the pH to 7.3.
2. Natural sea water is stored in the dark for at least three weeks to "age". If natural sea water is not available use artificial sea water.


## Artificial sea water

28.13	g	NaCl	
0.77	g	KCl	
1.6	g	CaCl2 x 2 H2O	
4.8	g	MgCl2 x 6 H2O	
0.11	g	NaHCO3	
3.5	g	MgSO4 x 7 H2O	
1000	ml	Distilled water