# 248: OAT FLAKES AGAR WITH YEAST EXTRACT


## Main sol. 248

20	g	Oat flakes	
1	ml	**Trace element solution**	
2	g	Yeast extract	
20	g	Agar	
1000	ml	Distilled water	

1. Adjust pH to 7.2.
2. Boil oat flakes for 20 min, filter through cheese cloth and adjust to 1000 ml. Add trace elements and agar.


## Trace element solution

0.1	g	FeSO4 x 7 H2O	
0.1	g	MnCl2 x 4 H2O	
0.1	g	ZnSO4 x 7 H2O	
100	ml	Distilled water