# 25: RHODOPSEUDOMONAS GLOBIFORMIS MEDIUM
## Main sol. 25
0.25 g Yeast extract
1.5 g Mannitol
0.5 g Na-gluconate
0.5 g KH2PO4
0.4 g MgSO4 x 7 H2O
0.4 g NH4Cl
0.4 g NaCl
0.05 g CaCl2 x 2 H2O
1 ml **Trace element solution SL-6**
1000 ml Distilled water
1. Adjust pH to 4.9, then add:
5 ml Fe(III) citrate (0.1% in H2O)
1 ml Biotin (0.002% in H2O)
1 ml p-Aminobenzoic acid (0.01% in H2O)
2 ml Sodium thiosulfate pentahydrate (10% in H2O)
2. Sterilize in screw-capped bottles. Cool to room temperature; to each 100 ml medium add
0.2 ml of a sterile 10% Na2S203 solution. Incubate in the light using a tungsten lamp
## Trace element solution SL-6
0.1 g ZnSO4 x 7 H2O
0.03 g MnCl2 x 4 H2O
0.3 g H3BO3
0.2 g CoCl2 x 6 H2O
0.01 g CuCl2 x 2 H2O
0.02 g NiCl2 x 6 H2O
0.03 g Na2MoO4 x 2 H2O
1000 ml Distilled water