# 25: RHODOPSEUDOMONAS GLOBIFORMIS MEDIUM ## Main sol. 25 0.25 g Yeast extract 1.5 g Mannitol 0.5 g Na-gluconate 0.5 g KH2PO4 0.4 g MgSO4 x 7 H2O 0.4 g NH4Cl 0.4 g NaCl 0.05 g CaCl2 x 2 H2O 1 ml **Trace element solution SL-6** 1000 ml Distilled water 1. Adjust pH to 4.9, then add: 5 ml Fe(III) citrate (0.1% in H2O) 1 ml Biotin (0.002% in H2O) 1 ml p-Aminobenzoic acid (0.01% in H2O) 2 ml Sodium thiosulfate pentahydrate (10% in H2O) 2. Sterilize in screw-capped bottles. Cool to room temperature; to each 100 ml medium add 0.2 ml of a sterile 10% Na2S203 solution. Incubate in the light using a tungsten lamp ## Trace element solution SL-6 0.1 g ZnSO4 x 7 H2O 0.03 g MnCl2 x 4 H2O 0.3 g H3BO3 0.2 g CoCl2 x 6 H2O 0.01 g CuCl2 x 2 H2O 0.02 g NiCl2 x 6 H2O 0.03 g Na2MoO4 x 2 H2O 1000 ml Distilled water