# 258: NUTRIENT STARCH AGAR ## Main sol. 258 5 g Peptone 3 g Meat extract 15 g Agar, if necessary 1 % Starch (soluble) 1000 ml Distilled water 1. Adjust pH to 7.0. For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.