# 266: THERMOCOCCUS CELER MEDIUM


## Main sol. 266

1.3	g	(NH4)2SO4	
0.28	g	KH2PO4	
0.25	g	MgSO4 x 7 H2O	
0.07	g	CaCl2 x 2 H2O	
0.02	g	FeCl3 x 6 H2O	
1.8	mg	MnCl2 x 4 H2O	
4.5	mg	Na2B4O7 x 10 H2O	
0.22	mg	ZnSO4 x 7 H2O	
0.05	mg	CuCl2 x 2 H2O	
0.03	mg	Na2MoO4 x 2 H2O	
0.03	mg	VOSO4 x 2 H2O	
0.01	mg	CoSO4 x 7 H2O	
40	g	NaCl	
1	mg	Resazurin	
2	g	Yeast extract	
5	g	Sulfur (powdered)	
1000	ml	Distilled water	

1. Adjust final pH to 5.8.
2. Prepare the medium anaerobically under 100% nitrogen. The following constituents are prepared separately and added to the autoclaved mineral salt solution: Yeast extract (20 ml of 10% w/v solution)-boiled for a few minutes not autoclaved; sulfur (10 g)-sterilized by steaming for 3 h on each of three successive days; Na2S x 9 H2O (10 ml of 3% w/v solution)-autoclaved under nitrogen atmosphere.