# 269: ACIDIPHILIUM MEDIUM


## Main sol. 269

2	g	(NH4)2SO4	
0.1	g	KCl	
0.5	g	K2HPO4	
0.5	g	MgSO4 x 7 H2O	
0.3	g	Yeast extract	
1	g	D-Glucose	
1000	ml	Distilled water	

1. Autoclave glucose and yeast extract separately as a 10% and 3%, resp., stock solution. Adjust pH of complete medium to 3.0 with 1 N H2SO4.