# 286: CLOSTRIDIUM AMINOBUTYRICUM MEDIUM


## Main sol. 286

1.3	g	KH2PO4	
7	g	K2HPO4	
0.2	g	MgCl2 x 6 H2O	
0.01	g	FeCl3 x 6 H2O	
0.01	g	CaCl2 x 2 H2O	
1	ml	Na2MoO4 x 2 H2O (0.1% w/v)	
3	g	Yeast extract	
5	g	gamma-Aminobutyric acid	
1.5	g	Agar	
0.5	ml	Sodium resazurin (0.1% w/v)	
0.3	g	Na2S x 9 H2O	
1000	ml	Distilled water	

1. Dissolve ingredients (except sulfide), adjust pH to 7.2, bring medium to the boil, then cool to room temperature under 100% N2 gas. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add sulfide from a sterile anoxic stock solution prepared under 100% N2 gas atmosphere.