# 312: SCY - MEDIUM ## Main sol. 312 1 g Sucrose 0.75 g Casitone 0.25 g Yeast extract 0.17 g Trypticase soy broth (without dextrose, BBL) 0.4 mg Thiamine-HCl x 2 H2O 0.01 mg Vitamin B12 1000 ml Distilled water 1. Adjust pH to 7.1. 2. Sterilize vitamins separately by filtration.