# 315: BACTEROIDES CELLULOSOLVENS MEDIUM


## Main sol. 315

0.68	g	NH4Cl	
0.3	g	K2HPO4	
0.18	g	KH2PO4	
0.15	g	(NH4)2SO4	
0.12	g	MgSO4 x 7 H2O	
0.06	g	CaCl2 x 2 H2O	
0.02	g	FeSO4 x 7 H2O	
10	ml	**Trace element solution**	
10	ml	**Vitamin solution**	
5	g	Cellobiose (cellulose: i.e. MN 300)	
1	mg	Resazurin	
2	g	NaHCO3	
0.25	g	L-Cysteine HCl x H2O	
0.25	g	Na2S x 9 H2O	
1000	ml	Distilled water	

1. Adjust pH to 7.0. Gas atmosphere: 80% N2 + 20% CO2.
2. Filter sterilize cellobiose separately.


## Trace element solution

1.5	g	Nitrilotriacetic acid	
3	g	MgSO4 x 7 H2O	
0.5	g	MnSO4 x H2O	
1	g	NaCl	
0.1	g	FeSO4 x 7 H2O	
0.18	g	CoSO4 x 7 H2O	
0.1	g	CaCl2 x 2 H2O	
0.18	g	ZnSO4 x 7 H2O	
0.01	g	CuSO4 x 5 H2O	
0.02	g	AlK(SO4)2 x 12 H2O	
0.01	g	H3BO3	
0.01	g	Na2MoO4 x 2 H2O	
0.025	g	NiCl2 x 6 H2O	
0.3	mg	Na2SeO3 x 5 H2O	
1000	ml	Distilled water	

1. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH).


## Vitamin solution

2	mg	Biotin	
2	mg	Folic acid	
10	mg	Pyridoxine hydrochloride	
5	mg	Thiamine-HCl x 2 H2O	
5	mg	Riboflavin	
5	mg	Nicotinic acid	
5	mg	D-Calcium pantothenate	
0.1	mg	Vitamin B12	
5	mg	p-Aminobenzoic acid	
5	mg	Lipoic acid	
1000	ml	Distilled water