# 351: RHODOBACTER ADRIATICUS MEDIUM


## Main sol. 351

0.82	g	Na acetate	
0.1	g	Yeast extract	
0.06	g	Na2S x 9 H2O	
1	g	K2HPO4	
0.1	g	CaCl2 x 2 H2O	
0.5	g	MgCl2 x 6 H2O	
1	g	NH4Cl	
3	g	NaHCO3	
25	g	NaCl	
0.5	g	Na-ascorbate	
1	ml	**Trace element solution (SLA)**	
1000	ml	Distilled water	
1	ml	**Vitamin solution (VA)**	

1. Adjust pH to 7.0.
2. The medium may be prepared without the addition of Na acetate, yeast extract and Na2S x 9H2O and may be added to the sterile medium from sterile stock solutions. Prepare the medium under an atmosphere of N2. Dispense into screw capped or crimp top bottles fitted with a rubber septum. Sterilize at 121°C for 15 minutes in screw-caped bottles. After sterilization add 1.0 ml/l filter-sterilized vitamin solution (VA, see below). Incubate at 30°C at a light intensity of 1500 lux under anaerobic conditions.


## Trace element solution (SLA)

1.8	g	FeCl2 x 4 H2O	
250	mg	CoCl2 x 6 H2O	
10	mg	NiCl2 x 6 H2O	
10	mg	CuCl2 x 2 H2O	
70	mg	MnCl2 x 4 H2O	
100	mg	ZnCl2	
500	mg	H3BO3	
30	mg	Na2MoO4 x 2 H2O	
10	mg	Na2SeO3 x 5 H2O	
1000	ml	Distilled water	

1. Adjust pH to 2.0 - 3.0.


## Vitamin solution (VA)

10	mg	Biotin	
35	mg	Nicotinic acid amide	
30	mg	Thiamine-HCl x 2 H2O	
20	mg	p-Aminobenzoic acid	
10	mg	Pyridoxal hydrochloride	
10	mg	Calcium pantothenate	
5	mg	Vitamin B12	
100	ml	Distilled water