# 354: LÖWENSTEIN-JENSEN MEDIUM


## Main sol. 354

2.5	g	KH2PO4	
0.24	g	MgSO4	
0.6	g	Mg-citrate	
3.6	g	L-Asparagin	
30	g	Potato flour	
0.4	g	Malachite green	
600	ml	Distilled water	

1. Addition: 

12	ml	Glycerol	
1000	ml	Fresh egg mixture (yolk and whites, *)	

2. pH 7.0
3. Sterilize medium for 30 min at 121°C, cool to 30°C and add fresh egg mixture carefully to the medium.
4. * You need for 1 l of egg mixture 20 - 25 fresh eggs (not older then 1 week). Clean the eggs, wipe away with 70% alcohol, open the eggs and homogenize the egg mixture carefully.