# 358: ACIDIANUS MEDIUM (AEROBIC)


## Main sol. 358

1.3	g	(NH4)2SO4	
0.28	g	KH2PO4	
0.25	g	MgSO4 x 7 H2O	
0.07	g	CaCl2 x 2 H2O	
0.02	g	FeCl3 x 6 H2O	
10	ml	**Allen's trace element solution**	
1	g	Yeast extract (OXOID)	
5	g	Sulfur (powder)	
1000	ml	Distilled water	

1. Dissolve ingredients, except sulfur, bring medium to the boil, then cool to room temperature and adjust pH to 2.5 using 10 N H2SO4. Dispense medium under air atmosphere enriched with 1 - 10% CO2 in culture vessels suitable for anaerobic cultivation (e.g., 20 ml medium in 100 ml serum bottles) containing already the appropriate amount of sulfur. For sterilization sealed bottles with medium are heated in a boiling water bath for 2 - 3 h on each of 3 successive days.
2. Note: Inoculate with 5% (w/v) culture. Incubate without shaking.


## Allen's trace element solution

180	mg	MnCl2 x 4 H2O	
450	mg	Na2B4O7 x 10 H2O	
22	mg	ZnSO4 x 7 H2O	
5	mg	CuCl2 x 2 H2O	
3	mg	Na2MoO4 x 2 H2O	
3	mg	VOSO4 x 2 H2O	
1	mg	CoSO4 x 7 H2O	
1000	ml	Distilled water	

1. Adjust pH of final solution to 2 with 1 N HCl.