# 36: THIOBACILLUS MEDIUM


## Main sol. 36

0.1	g	(NH4)2SO4	
4	g	K2HPO4	
4	g	KH2PO4	
0.1	g	MgSO4 x 7 H2O	
0.1	g	CaCl2	
0.02	g	FeCl3 x 6 H2O	
0.02	g	MnSO4 x H2O	
12	g	Agar, for solid medium (optional)	
10	g	Na2S2O3 x 5 H2O	
1000	ml	Distilled water	

1. Dissolve ingredients except thiosulfate and adjust the pH to 6.6. Dispense in suitable culivation vials and autoclave. Add thiosulfate to the autoclaved medium from a sterile stock solution.