# 368: KUNKEE MEDIUM ## Main sol. 368 20 g Tryptone 5 g Peptone 5 g Yeast extract 5 g Glucose 0.5 ml Tween 80 250 ml Tomato juice (filtered ) 750 ml Distilled water 1. Adjust pH to 5.5. 2. After sterilization aseptically add 10% (v/v) ethanol