# 38: ACETOANAEROBIUM MEDIUM ## Main sol. 38 20 g Tryptone 10 g Yeast extract 1.04 g K2HPO4 0.68 g KH2PO4 0.5 ml Sodium resazurin (0.1% w/v) 0.15 g Na2S x 9 H2O 1000 ml Distilled water 1. Dissolve ingredients (except sulfide), adjust pH to 7.0, bring medium to the boil, then cool to room temperature under 100% N2 gas. Dispense under same gas atmosphere in culture vessels and autoclave. After sterilization add sulfide from a sterile anoxic stock solution prepared under 100% N2 gas.