# 388: DICTYOGLOMUS MEDIUM


## Main sol. 388

1.5	g	KH2PO4	
4.2	g	Na2HPO4 x 12 H2O	
0.5	g	NH4Cl	
0.38	g	MgCl2 x 6 H2O	
0.06	g	CaCl2 x 2 H2O	
0.04	g	Fe(NH4)2(SO4)2 x 6 H2O	
2.9	ml	CoCl2 x 6 H2O (0.1% w/v)	
2.4	ml	Na2MoO4 x 2 H2O (0.1% w/v)	
1.7	ml	Na2SeO3 x 5 H2O (0.01% w/v)	
2	ml	MnCl2 x 4 H2O (0.1% w/v)	
2.8	ml	ZnSO4 (0.1% w/v)	
2	g	Yeast extract	
2	g	Polypeptone	
5	g	Starch (soluble)	
0.5	ml	Sodium resazurin (0.1% w/v)	
1	g	Na2CO3	
1	g	L-Cysteine HCl x H2O	
1	ml	**Wolin's vitamin solution (10x)**	
1000	ml	Distilled water	

1. Dissolve ingredients (except carbonate, cysteine and vitamins), bring medium to the boil, then cool to room temperature under 100% N2 gas atmosphere. Add and dissolve carbonate and cysteine, adjust pH to 7.2, then dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add vitamins from an anoxic stock solution prepared under 100% N2 gas and sterilized by filtration. Adjust pH of complete medium to 7.2, if necessary.


## Wolin's vitamin solution (10x)

20	mg	Biotin	
20	mg	Folic acid	
100	mg	Pyridoxine hydrochloride	
50	mg	Thiamine HCl	
50	mg	Riboflavin	
50	mg	Nicotinic acid	
50	mg	Calcium D-(+)-pantothenate	
1	mg	Vitamin B12	
50	mg	p-Aminobenzoic acid	
50	mg	(DL)-alpha-Lipoic acid	
1000	ml	Distilled water