# 414: ACIDAMINOCOCCUS FERMENTANS MEDIUM ## Main sol. 414 5 g Trypticase peptone 10 g Casamino acids 5 g Yeast extract 5 g Glucose 4 g Na glutamate 1 g Arginine 1 g Glycine 0.1 g DL-Tryptophan 2 g KH2PO4 0.5 ml Tween 80 0.5 g L-Cysteine HCl x H2O 1000 ml Distilled water 1. pH 7.0 - 7.2. 2. Prepare and distribute medium anaerobically using the Hungate technique