# 429c: COLUMBIA-GLUCOSE-CYSTEINE-AGAR


## Main sol. 429c

1000	ml	Columbia agar base	
4	%	Horse blood	
1	%	Glucose	
0.1	%	Cysteine	

1. Mix autoclaved Columbia agar base solution with 4% horse or sheep blood. After cooling to 50°C add 1% glucose and 0.1% cysteine, mix well and pour plates. Rehydrate pellets of freeze-dried ampoules in liquid medium 220 or 215.


## Main sol. 220

15	g	Casein peptone	
5	g	Soy peptone	
5	g	NaCl	
15	g	Agar	
1000	ml	Distilled water	

1. Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid CM 131).


## Main sol. 215

37	g	Brain heart infusion (Difco)	
1000	ml	Distilled water