# 429d: FRANCISELLA HALIOTICIDA MEDIUM


## Main sol. 429d

1000	ml	Columbia agar base (dissolved in 70 % artificial seawater)	
2	g/l	Agar	
4	%	Horse blood	
1	%	Glucose	
2	mM	FeCl3	
0.1	%	Cysteine	

1. Dissolve Columbia agar base in 70% artificial seawater and add additional 2 g agar per liter. Autoclave and mix solution with 4% horse or sheep blood after cooling to 50°C. Add 1% glucose, 2 mM FeCl3 and 0.1% cysteine, mix well and pour plates. Rehydrate pellets of freeze-dried ampoules in liquid medium 220 or 215.


## Main sol. 220

15	g	Casein peptone	
5	g	Soy peptone	
5	g	NaCl	
15	g	Agar	
1000	ml	Distilled water	

1. Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid CM 131).


## Main sol. 215

37	g	Brain heart infusion (Difco)	
1000	ml	Distilled water