# 452: GLUCOSE MEDIUM (NAKAYAMA)


## Main sol. 452

10	g	Glucose	
10	g	Peptone	
15	g	Yeast extract	
10	g	Agar	
980	ml	Distilled water	
10	ml	**Solution A**	
10	ml	**Solution B**	
1	ml	**Solution C**	

1. Autoclave all solutions separately, final pH is 6.8.


## Solution A

0.5	g	KH2PO4	
0.5	g	K2HPO4	
100	ml	Distilled water	


## Solution B

3	g	MgSO4 x 7 H2O	
0.1	g	NaCl	
0.1	g	MnSO4 x 5 H2O	
0.01	g	CuSO4 x 5 H2O	
100	ml	Distilled water	


## Solution C

0.1	g	FeSO4 x 7 H2O	
2	g	Na3-citrate	
100	ml	Distilled water