# 456: KPL MEDIUM


## Main sol. 456

1000	ml	**Lactic acid whey**	
140	ml	**White table wine**	
10	g	Glucose	
10	g	Galactose	
1	ml	Tween 80	
15	g	Agar	

1. Adjust pH to 5.5


## Lactic acid whey

10	%	Skim milk	

1. Adjust 10% skim milk to pH 5.5 with lactic acid and heat at 100°C for 30 minutes. Remove precipitate by filtration. For preparing broth medium, adjust filtrate to pH 7.0 with 2 N NaOH and heat at 100°C for 30 minutes. Remove precipitate by filtration. Adjust filtrate to pH 5.5 with 2 N HCl.


## White table wine

200	ml	White wine	

1. Filter-sterilize and add to autoclaved basal medium.