# 458: MEDIUM FOR BACILLUS STEAROTHERMOPHILUS


## - PHENOL DEGRADING

0.5	g	K2HPO4	
1	g	NH4Cl	
0.02	g	MgSO4 x 7 H2O	
0.2	g	Yeast extract	
0.1	g	Casamino acids	
900	ml	Distilled water	

1. pH 7.4
2. Autoclave for 15 min. Add 1 ml of sterile SL-4 (see medium 14) and 100 ml of filter-sterilized phenol-solution (4.7g/l).

1	ml	**Trace element solution SL-4**	
100	ml	Phenol (filter-sterilized, 4.7 g/l)	


## Trace element solution SL-4

0.5	g	Na2-EDTA	
0.2	g	FeSO4 x 7 H2O	
0.1	g	ZnSO4 x 7 H2O	
0.03	g	MnCl2 x 4 H2O	
0.3	g	H3BO3	
0.2	g	CoCl2 x 6 H2O	
0.01	g	CuCl2 x 2 H2O	
0.02	g	NiCl2 x 6 H2O	
0.03	g	Na2MoO4 x 2 H2O	
1000	ml	Distilled water	

1. First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds.