# 466: TSBY SALT MEDIUM


## Main sol. 466

30	g	Trypticase soy broth	
3	g	Yeast extract	
13	g	NaCl	
0.34	g	KCl	
4	g	MgCl2 x 6 H2O	
3.45	g	MgSO4 x 7 H2O	
0.25	g	NH4Cl	
0.14	g	CaCl2 x 2 H2O	
1000	ml	Distilled water	

1. Adjust pH to 7.2
2. Incubate under microaerophilic conditions