# 470: PSEUDOMONAS HALOPHILA MEDIUM


## Main sol. 470

900	ml	**Solution A**	
100	ml	**Solution B**	

1. Vitamin solution is filter-sterilized and added after autoclaving. Solution A and B are autoclaved separately and combined after cooling.
2. Adjust pH to 7.0 with 6 M NaOH or HCl.


## Solution A

46.8	g	NaCl	
39.4	g	MgSO4 x 7 H2O	
1	g	NH4Cl	
5	g	Glycerol	
1	ml	**Trace element solution SL-10**	
10	ml	**Vitamin solution**	
890	ml	Distilled water	


## Solution B

1	g	KH2PO4	
100	ml	Distilled water	


## Trace element solution SL-10

10	ml	HCl (25%)	
1.5	g	FeCl2 x 4 H2O	
70	mg	ZnCl2	
100	mg	MnCl2 x 4 H2O	
6	mg	H3BO3	
190	mg	CoCl2 x 6 H2O	
2	mg	CuCl2 x 2 H2O	
24	mg	NiCl2 x 6 H2O	
36	mg	Na2MoO4 x 2 H2O	
990	ml	Distilled water	

1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.


## Vitamin solution

2	mg	Biotin	
2	mg	Folic acid	
10	mg	Pyridoxine hydrochloride	
5	mg	Thiamine-HCl x 2 H2O	
5	mg	Riboflavin	
5	mg	Nicotinic acid	
5	mg	Calcium pantothenate	
1	mg	p-Aminobenzoic acid	
0.01	mg	Vitamin B12	
1000	ml	Distilled water