# 471: ALCALIGENES XYLOSOXYDANS MEDIUM WITH BENZOATE


## Main sol. 471

500	ml	**Solution A**	
500	ml	**Solution B**	
2	ml	**Solution C**	

1. Autoclave solutions A, B and C separately, combine, adjust pH to 8.2.


## Solution A

5.12	g	K2HPO4	
1.5	g	KH2PO4	
500	ml	Distilled water	

1. Adjust pH to 9.0 with KOH.


## Solution B

0.3	g	NH4Cl	
0.01	g	CaCl2	
0.2	g	MgSO4 x 7 H2O	
2	g	KNO3	
0.92	g	Na-benzoate	
500	ml	Distilled water	


## Solution C

2	ml	**Trace element solution SL-4**	


## Trace element solution SL-4

0.5	g	EDTA	
0.2	g	FeSO4 x 7 H2O	
100	ml	**Trace element solution SL-6**	
900	ml	Distilled water	

1. First dissolve EDTA in distilled water by adjusting the pH to 7.0 - 8.0 using a 2 M solution of NaOH; then add ferrous sulfate and the trace element solution SL-6. 
2. First dissolve EDTA in distilled water by adjusting the pH to 7.0 - 8.0 using a 2 M solution
of NaOH; then add ferrous sulfate and the trace element solution SL–6.


## Trace element solution SL-6

0.1	g	ZnSO4 x 7 H2O	
0.03	g	MnCl2 x 4 H2O	
0.3	g	H3BO3	
0.2	g	CoCl2 x 6 H2O	
0.01	g	CuCl2 x 2 H2O	
0.02	g	NiCl2 x 6 H2O	
0.03	g	Na2MoO4 x 2 H2O	
1000	ml	Distilled water