# 477: ACIDIHALOBACTER PROSPERUS MEDIUM


## Main sol. 477

0.33	g	KCl	
2.75	g	MgCl2 x 6 H2O	
3.45	g	MgSO4 x 7 H2O	
1.25	g	NH4Cl	
0.14	g	CaCl2 x 2 H2O	
0.14	g	K2HPO4	
0.14	g	KH2PO4	
0.5	g	NaCl	
10	ml	**Modified Wolin's mineral solution**	
2	ml	NiCl2 x 6 H2O (0.1% w/v)	
50	ml	FeSO4 x 7 H2O (40% w/v)	
940	ml	Distilled water	

1. Dissolve ingredients except ferrous sulfate and adjust pH to 2.5 with 4 N H2SO4. Sterilize medium by autoclaving for 15 min at 121°C. Prior to inoculation add FeSO4 x 7 H2O from a 40% (w/v) stock solution prepared with 0.2 N H2SO4 and filter-sterilized under 100% N2 gas atmosphere.


## Modified Wolin's mineral solution

1.5	g	Nitrilotriacetic acid	
3	g	MgSO4 x 7 H2O	
0.5	g	MnSO4 x H2O	
1	g	NaCl	
0.1	g	FeSO4 x 7 H2O	
0.18	g	CoSO4 x 7 H2O	
0.1	g	CaCl2 x 2 H2O	
0.18	g	ZnSO4 x 7 H2O	
0.01	g	CuSO4 x 5 H2O	
0.02	g	AlK(SO4)2 x 12 H2O	
0.01	g	H3BO3	
0.01	g	Na2MoO4 x 2 H2O	
0.03	g	NiCl2 x 6 H2O	
0.3	mg	Na2SeO3 x 5 H2O	
0.4	mg	Na2WO4 x 2 H2O	
1000	ml	Distilled water	

1. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.