# 48: TRYPTONE THIOGLYCOLATE MEDIUM


## Main sol. 48

5.45	g	K2HPO4	
1.2	g	KH2PO4	
25	mg	MgSO4 x 7 H2O	
15	mg	CaCl2 x 2 H2O	
10	mg	FeSO4 x 7 H2O	
2	mg	MnCl2 x 4 H2O	
2.5	mg	CoCl2 x 6 H2O	
2.5	mg	Na2MoO4 x 2 H2O	
20	g	Glucose	
2	g	Peptone	
2	g	Tryptone	
6	g	Yeast extract	
0.5	g	Na-thioglycolate	
1000	ml	Distilled water	

1. Dissolve ingredients (except glucose), adjust pH to 7.5 and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave.
2. Add glucose to the medium after autoclaving from a sterile stock solution prepared under 100% N2 gas.