# 514: BACTO MARINE BROTH (DIFCO 2216)


## Main sol. 514

5	g	Bacto peptone	
1	g	Yeast extract (Bacto )	
0.1	g	Fe(III) citrate	
19.45	g	NaCl	
5.9	g	MgCl2 (anhydrous)	
3.24	g	Na2SO4	
1.8	g	CaCl2	
0.55	g	KCl	
0.16	g	NaHCO3	
0.08	g	KBr	
34	mg	SrCl2	
22	mg	H3BO3	
4	mg	Na-silicate	
2.4	mg	NaF	
1.6	mg	(NH4)NO3	
8	mg	Na2HPO4	
1000	ml	Distilled water	

1. Final pH should be 7.6 ± 0.2 at 25°C. If using the complete medium from Difco add 37.40 g to 1 litre water.
2. Note: M.514 refers to Bacto Marine Broth Difco 2216 (BD 279110). Other brands (even if they refer to Difco2216) have not been tested and might not support growth for some strains.