# 514b: MEDIUM 514 plus additional salt ## Main sol. 514b 1000 ml **Main sol. 514** 17.5 g/l Agar (adjust in required in range 15-20), for solid medium 1. Use medium 514 to which is added NaCl at the concentration indicated (i.e. "514 + 6% NaCl" indicates use medium 514 to which 60 g/l NaCl has been added; see strain dependant modifications). 2. Final pH 7.6. The medium may be solidified by adding 15 - 20 g/l agar. ## Main sol. 514 5 g Bacto peptone 1 g Yeast extract (Bacto ) 0.1 g Fe(III) citrate 19.45 g NaCl 5.9 g MgCl2 (anhydrous) 3.24 g Na2SO4 1.8 g CaCl2 0.55 g KCl 0.16 g NaHCO3 0.08 g KBr 34 mg SrCl2 22 mg H3BO3 4 mg Na-silicate 2.4 mg NaF 1.6 mg (NH4)NO3 8 mg Na2HPO4 1000 ml Distilled water 1. Final pH should be 7.6 ± 0.2 at 25°C. If using the complete medium from Difco add 37.40 g to 1 litre water.