# 514e: MARINE BROTH (ANAEROB)


## Main sol. 514e

5	g	Peptone (BD Bacto)	
1	g	Yeast extract (BD Bacto)	
19.45	g	NaCl	
5.9	g	MgCl2 (anhydrous)	
3.24	g	Na2SO4	
0.55	g	KCl	
0.16	g	NaHCO3	
0.08	g	KBr	
34	mg	SrCl2	
22	mg	H3BO3	
4	mg	Na-silicate	
2.4	ml	NaF (0.1% w/v)	
1.6	ml	(NH4)NO3 (0.1% w/v)	
8	ml	Na2HPO4 (0.1% w/v)	
0.5	ml	Sodium resazurin (0.1% w/v)	
0.3	g	L-Cysteine HCl x H2O	
0.3	g	Na2S x 9 H2O	
1000	ml	Distilled water	

1. Dissolve ingredients except cysteine and sulfide, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Prior to use check pH of complete medium and adjust to 7.3 - 7.5, if necessary.