# 514f: MARINE BROTH (ROTH CP73)


## Main sol. 514f

5	g	Peptone	
1	g	Yeast extract	
0.1	g	Fe(III) citrate	
19.4	g	NaCl	
8.8	g	MgCl2	
3.24	g	Na2SO4	
1.8	g	CaCl2	
0.55	g	KCl	
0.16	g	NaHCO3	
0.08	g	KBr	
34	mg	SrCl2	
22	mg	H3BO3	
4	mg	Na-silicate	
2.4	mg	NaF	
1.6	mg	(NH4)NO3	
8	mg	Na2HPO4	
1000	ml	Distilled water	
15	g/l	Agar, if required	

1. Final pH should be 7.6 ± 0.2 at 25°C. Add 15.0 g/l agar for solid media. We recommend using the medium supplied by Carl Roth GmbH + Co. KG for Marinomonas mediterranea DSM 23531.