# 514g: HALF STRENGTH BACTO MARINE BROTH with 50% Seawater


## Main sol. 514g

2.5	g	Bacto peptone	
0.5	g	Yeast extract (Bacto )	
0.05	g	Fe(III) citrate	
9.725	g	NaCl	
2.95	g	MgCl2 (anhydrous)	
1.62	g	Na2SO4	
0.9	g	CaCl2	
0.275	g	KCl	
0.08	g	NaHCO3	
0.04	g	KBr	
17	mg	SrCl2	
11	mg	H3BO3	
2	mg	Na-silicate	
1.2	mg	NaF	
0.8	mg	(NH4)NO3	
4	mg	Na2HPO4	
500	ml	Sea water	
500	ml	Distilled water	
15	g/l	Agar, for solid medium	

1. Final pH should be 7.6 ± 0.2 at 25°C. If using Difco Bacto Marine Broth use 18.7 g/l to 1 litre water.
2. The medium may be solidified by adding 15 g/l agar.