# 529: AMPHIBACILLUS MEDIUM


## Main sol. 529

10	g	Glucose	
1	g	K2HPO4	
2	g	NH4NO3	
0.2	g	MgSO4 x 7 H2O	
5	mg	MnSO4 x 7 H2O	
5	mg	FeSO4 x 7 H2O	
0.1	g	CaCl2 x 2 H2O	
3	g	Yeast extract	
0.3	g	Polypeptone (BBL)	
15	g	Agar	
900	ml	Distilled water	
100	ml	**Na-sesquicarbonate solution**	

1. After sterilization add sterile 1 M Na-sesquicarbonate solution (1 ml in 10 ml) to achieve a pH of 9.7 (see medium 31).


## Na-sesquicarbonate solution

4.2	g	NaHCO3	
5.3	g	Na2CO3 anhydrous (anhydrous)	
100	ml	Distilled water