# 530: THIOGLYCOLATE MEDIUM


## Main sol. 530

5	g	Casein peptone	
3	g	Meat extract	
0.5	g	Na-thioglycolate	
15	g	Agar	
1000	ml	Distilled water	

1. Adjust pH to 5.5.
2. Sterilize Na-Thioglycolate separately by filtration.
3. Optimal pO2 for growth on solid media is 0.03 - 0.08 atm.