# 535a: TRYPTICASE SOY BROTH AGAR+BLOOD


## Main sol. 535a

1000	ml	**Main sol. 535**	
5	%	Sheep blood	

1. To medium 535 add 5% blood after cooling.


## Main sol. 535

30	g	Trypticase soy broth (BBL 11768, Oxoid CM129 or Merck 5459)	
20	g	Agar	
1000	ml	Distilled water	

1. pH 7.3
2. Autoclave at 121°C for 15 min.