# 545a: DILUTED TRYPTONE SOYA BROTH (TSB)


## Main sol. 545a

10	ml	**Main sol. 545**	
990	ml	Distilled water	

1. Dilute medium 545 1:10.


## Main sol. 545

17	g	Casein peptone	
3	g	Soy peptone	
2.5	g	D(+)-Glucose	
5	g	NaCl	
2.5	g	K2HPO4	
1000	ml	Distilled water	

1. Adjust pH to 7.3.
2. The same as Caso Bouillon (Merck or Oxoid, Germany).