# 546: LC BROTH MEDIUM


## Main sol. 546

10	g	Tryptone	
5	g	Yeast extract	
10	g	NaCl	
1	g	Glucose	
990	ml	Distilled water	
5	ml	CaCl2 x 2 H2O (1 M)	
5	ml	MgSO4 x 7 H2O (1 M)	

1. Adjust pH to 7.4.
2. Sterilize at 121°C for 15 min. Cool to 50°C and add 5 ml 1 M CaCl2 x 2 H2O and 5 ml 1 M MgSO4 x 7 H2O as sterile solutions.