# 54a: REACTIVATION WITH LIQUID MEDIUM 545


## Main sol. 54a

1000	ml	**54. GLUCOSE YEAST EXTRACT AGAR**	
1000	ml	**545. TRYPTONE SOYA BROTH (TSB)**	

1. Rehydrate and grow lyophilized cells from the ampoule in liquid medium 54 or 545. Subsequent subculturing may be carried out in liquid medium or on agar plates medium 54.


## 54. GLUCOSE YEAST EXTRACT AGAR

20	g	Glucose	
10	g	Yeast extract	
20	g	CaCO3 (light precipitate)	
17	g	Agar, for solid medium	
1000	ml	Distilled water	


## 545. TRYPTONE SOYA BROTH (TSB)

17	g	Casein peptone	
3	g	Soy peptone	
2.5	g	D(+)-Glucose	
5	g	NaCl	
2.5	g	K2HPO4	
1000	ml	Distilled water	

1. Adjust pH to 7.3.
2. The same as Caso Bouillon (Merck or Oxoid, Germany).