# 55: FRUCTOSE MINERAL MEDIUM


## Main sol. 55

500	ml	**Solution A**	
300	ml	**Solution B**	
200	ml	**Solution C**	
1.5	%	Agar, if required	

1. Autoclave solutions A and B separately, filter sterilize solution C. Add 1.5% agar if necessary.


## Solution A

9	g	Na2HPO4 x 12 H2O	
1.5	g	KH2PO4	
1	g	NH4Cl	
0.2	g	MgSO4 x 7 H2O	
0.3	ml	**Trace element solution SL-6**	
500	ml	Distilled water	


## Solution B

0.01	g	CaCl2 x 2 H2O	
5	mg	Ferric ammonium citrate	
300	ml	Distilled water	


## Solution C

5	g	Fructose	
200	ml	Distilled water	


## Trace element solution SL-6

0.1	g	ZnSO4 x 7 H2O	
0.03	g	MnCl2 x 4 H2O	
0.3	g	H3BO3	
0.2	g	CoCl2 x 6 H2O	
0.01	g	CuCl2 x 2 H2O	
0.02	g	NiCl2 x 6 H2O	
0.03	g	Na2MoO4 x 2 H2O	
1000	ml	Distilled water