# 573: THIOTHRIX MEDIUM


## Main sol. 573

0.2	g	NH4Cl	
0.01	g	K2HPO4	
0.01	g	MgSO4 x 7 H2O	
20	ml	CaSO4 (saturated solution)	
5	ml	**Trace element solution**	
0.1	g	Na-acetate	
12	g	Agar, if required	
1000	ml	Distilled water	

1. Adjust pH to 7.5 before autoclaving. Sterilize separately a 10% (w/v) solution of Na2S x 9 H2O; add the following amount of this solution to the medium after autoclaving (= shortly before using the medium).

3	ml	**neutralized Na2S x 9 H2O solution, 10% (w/v)**	

2. Note: For small volumes, such as 5 mL agar slants, you can use the respective volume of a sterile neutralized 3% Na2S x 9 H2O solution (e.g., 50µL/5 mL = 0.3g/L)


## Trace element solution

0.2	g	EDTA	
0.7	g	FeSO4 x 7 H2O	
0.01	g	ZnSO4 x 7 H2O	
2	mg	MnSO4 x 4 H2O	
5	µg	CuSO4 x 5 H2O	
10	mg	H3BO3	
1	mg	Co(NO3)2	
1	mg	Na2MoO4 x 2 H2O	
1000	ml	Distilled water	


## neutralized Na2S x 9 H2O solution, 3% (w/v)

3	%(w/v)	Na2S x 9 H2O	
1000	ml	Distilled water	

1. Adjust pH to 7.0.


## neutralized Na2S x 9 H2O solution, 10% (w/v)

10	g	Na2S x 9 H2O	
100	ml	Distilled water	

1. Adjust pH to 7.0.