# 58: BIFIDOBACTERIUM MEDIUM


## Main sol. 58

10	g	Casein peptone (tryptic digest)	
5	g	Yeast extract	
5	g	Meat extract	
5	g	Bacto Soytone	
10	g	Glucose	
2	g	K2HPO4	
0.2	g	MgSO4 x 7 H2O	
0.05	g	MnSO4 x H2O	
1	ml	Tween 80	
5	g	NaCl	
0.5	g	L-Cysteine HCl x H2O	
40	ml	**Salt solution**	
4	ml	Resazurin (25 mg/100ml)	
950	ml	Distilled water	

1. The cysteine are added after the medium has been boiled and cooled under CO2. Adjust pH to 6.8 using 8 N NaOH. Distribute under N2 and autoclave.


## Salt solution

0.25	g	CaCl2 x 2 H2O	
0.5	g	MgSO4 x 7 H2O	
1	g	K2HPO4	
1	g	KH2PO4	
10	g	NaHCO3	
2	g	NaCl	
1000	ml	Distilled water