# 582: 15% MH MEDIUM


## Main sol. 582

121.5	g	NaCl	
10.5	g	MgCl2	
14.4	g	MgSO4	
0.54	g	CaCl2	
3	g	KCl	
0.09	g	NaHCO3	
0.039	g	NaBr	
5	g	Proteose peptone no. 3 (Difco)	
10	g	Yeast extract (Difco)	
1	g	Glucose	
1000	ml	Distilled water	

1. Final pH 7.5
2. NaHCO3 is added from a sterilized stock solution when the medium has cooled. Agar is added at a concentration of 20 g/l for solid media.